agrisera品牌试剂PR-1 发病机理相关蛋白1
background Pathogenesis-related protein 1 (PR-1) is partially responsible for acquired pathogen resistance. Induced by INA, salicylic acid and pathogen infection.
This product is a recombinant PR-1protein, trunctated by first 26 amino acids, source:Arabidopsis thaliana,UniProt:P33154, TAIR: At2g14610
antibody format
does not apply
quantity
100µl, lyophilized, for reconstitution add 90 µl of milliQ water.
Please notice that this product contains 10% glycerol and might
appear as liquid but is provided lyophilized.
storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications western blot (WB)
related products AS10 687 | rabbit antibody
collection of antibodies to other proteins involved in a response to pathogen attack
additional information The PR-1 protein standard can be used in combination with anti-PR-1 antibodies to quantitate PR-1 protein. Quantitative western blot: detailed method description, video tutorial
APPLICATION INFORMATION
recommended dilution Standard curve: 3 loads are recommended eg.0.5, 2 and 4μl.
For most applications a sample load of 10-20 μg of protein will provide with a signal in this range.
Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.
expected | apparent MW 16.4 kDa
additional information Concentration: after adding 90 µl of sterile milliQ water final concentration of the standard is 0.10 pmoles/µl
Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.
This standard is ready-to-load and does not require any additions or heating after rehydratation.
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